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US 5,348,857 AGranted
Probes and method for identifying species and biovars of brucella
AssigneeTexas A&M University
InventorsThomas A. Ficht, L. Garry Adams
Life of this patent
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Abstract
A method for detecting Brucella infection in an animal which is reliable, rapid, and able to identify species and biovars of Brucella. The detection method includes the amplification of the omp2 gene locus of Brucella and analysis of restriction digestion fragments specific to Brucella and to individual species and groups of biovars of Brucella.
Claims13
A method for identifying a species or biovar of Brucella comprising the steps of:
- releasing DNA from a test sample;
- amplifying a gene sequence of a Brucella omp2 gene locus as defined in FIGS. 2A-O from the released DNA, said gene sequence having sufficient diversity to distinguish species of Brucella;
- analyzing the hybridization of the amplified gene sequence with a panel of DNA probes to identify a species or biovar of Brucella.
Dependencies are inferred from the claim text.