Dual energy tracer quantitative analysis

A method for quantitative analysis of plural target substances existing on a substrate comprising,

• tagging first and second target substances on a substrate with first and second radioactive labels, while also tagging a series of standards on the substrate with the same labels, the first label having a more energetic radiation emission characteristic than the second label, radiation emitted by the labels having the property of activating storage phosphor material,
• exposing a first storage phosphor screen to said substrate, thereby creating a first latent image on the first storage phosphor screen,
• exposing a second storage phosphor screen to said substrate through a radiation emission attenuating membrane, said membrane attenuating substantially more of the less energetic radiation from the target substance tagged with the second label than the more energetic radiation from the target substance tagged with the first label, thereby creating a second latent image on the second storage phosphor screen with a relatively larger intensity contribution from the first label than the that from the second label as compared to corresponding contributions to the latent image on the first storage phosphor screen,
• developing the latent images by scanning the storage phosphor screens,
• producing signal representations of the developed images wherein exposure efficiencies of said labels are substantially constants in the image representations,
• calculating the exposure efficiency for each label in each of said exposures from those portions of the image representations corresponding to said set of standards for each label and from an independent measurement of radiation emitted from each standard on said substrate,
• measuring integrated image intensity per spot area on each image representation,
• simultaneously determining the amount of each target substance in the spot area using the image intensities and exposure efficiencies.

A method for quantitative analysis of two target substances existing on a substrate comprising:

• tagging first and second target substances on a substrate with primary and secondary labels, respectively, the primary label having a more energetic radiation emission characteristic than the secondary label, radiation emitted by the labels having the property of activating storage phosphor material,
• providing the substrate with a set of standards for each label,
• exposing a radiation storage phosphor material to said substrate containing said tagged target substances and said standards, thereby creating a first latent image on said radiation storage phosphor material,
• developing said first latent image to produce a first image pattern,
• storing said first image pattern in a storage phosphor material,
• erasing said first latent image from said radiation storage phosphor material,
• exposing said radiation storage phosphor material to said substrate through a radiation emission attenuating membrane, said membrane attenuating substantially more of the less energetic radiation from the target substance tagged with the secondary label than the more energetic radiation from the target substance tagged with the primary label, thereby creating a second latent image on said radiation storage phosphor material with a relatively larger intensity contribution from the primary label than that from the secondary label as compared to corresponding contributions to the first latent image,
• developing said second latent image to produce a second image pattern,
• storing said second image pattern in said storage phosphor material,
• constructing an image intensity curve from each of said stored image patterns, such that exposure efficiencies of said labels are substantially constants in said curves,
• calculating the exposure efficiency, E, for each label under both exposuring steps from those portions of said image intensity curves corresponding to said set of standards for each label and from an independent measurement of radiation emitted from each standard on said substrate, yielding four efficiencies, E.sub.1P, E.sub.2P, E.sub.1S, E.sub.2S where
• E.sub.1P =Efficiency for primary label in first radiation storage phosphor material exposing step
• E.sub.2P =Efficiency for primary label in second radiation storage phosphor material exposing step
• E.sub.1S =Efficiency for secondary label in first radiation storage phosphor material exposing step
• E.sub.2S =Efficiency for secondary label in second radiation storage phosphor material exposing step;
• measuring image intensity per spot area on each image intensity curve as concentrations C.sub.1 and C.sub.2 ;
• and
• solving the two equations for D.sub.P and D.sub.S
• C.sub.1 =E.sub.1P D.sub.P +E.sub.1S D.sub.S
• C.sub.2 =E.sub.2P D.sub.P +E.sub.2S D.sub.S
• where D.sub.P and D.sub.S is the total radiation per label at said spot area, the total radiation per label indicating the amount of each of the two tagged target substances on the substrate.