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AssigneePasteur Merieux Serums et Vaccins
InventorsMichel G. J. Grandgeorge, Jean-Luc B. Veron, Pierre L. J. Fournier
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The process for isolating albumin from supernatant IV, and in particular IV-4, or from COHN's fraction V or from a plasma supernatant or fraction of analogous composition derived from an alcoholic or nonalcoholic fractionation, comprises one step on a hydrophilic anion exchange column with binding of albumin to the column, and then elution, and one step on a hydrophobic anion exchange column. Albumin thus obtained having a purity >99% by cellulose acetate electrophoresis, being free from impurities detected by crossed immunoelectrophoresis and free from polymers.
A process for isolating albumin having a purity greater than 99% with a polymer content lower than 1%, from a supernatant IV, or from COHN's fraction V derived from an alcoholic fractionation, comprising two anion exchange chromatographic steps, wherein one step is carried out on a hydrophilic anion exchange column with binding of albumin to the column followed by elution, and the other step is carried out on a hydrophobic anion exchange column.
The process as claimed in claim 7, wherein the hydrophilic polymer is a dextran derivative and the hydrophobic polymer is a polyvinyltoluene derivative.
A process for isolating albumin having a purity essentially of 100% with a polymer content essentially of 0%, from a supernatent IV, or from COHN's fraction V, comprising two anion exchange chromatographic steps, wherein one step is carried out on a hydrophilic anion exchange column with binding of albumin to the column, followed by elution, and the other is carried out on a hydrophobic anion exchange column.
Dependencies are inferred from the claim text.