Process for obtaining cellular protein having anti-HIV activity
AssigneeTampa Bay Research Institute
InventorsMeihan Nonoyama, Akiko Tanaka, Patrick K. Lai
Life of this patent
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Abstract
A process for obtaining cellular protein having potent anti-HIV activity comprises culturing CD4-positive T cells or myeloid cells in a tissue culture medium under conditions in which the cells are induced to produce and secrete a protein having anti-HIV activity, separating the cells from the culture medium containing the protein and then recovering the protein from the medium, wherein the molecular weight of the protein having anti-HIV activity, as measured by gel filtration analysis, is within the range of about 7,000 to about 12,000 daltons.
Claims19
A method for obtaining a cellular protein having anti-HlV activity which comprises:
- (a) culturing CD4-positive T cells in a tissue culture medium under conditions in which the cells are induced to produce and secrete at least one protein soluble in the culture medium having anti-HIV activity,
- (b) separating the cells from the culture medium containing the protein having anti-HIV activity, and
- (c) recovering the protein from the medium, wherein the protein is characterized by a molecular weight of about 7,000 to about 12,000 daltons as determined by gel filtration analysis.
A method for obtaining a cellular protein having anti-HIV activity which comprises:
- (a) culturing myeloid cells in a tissue culture medium comprising PC6, alkaline lignin, de-alkaline lignin or lignin sulfonate under conditions in which the cells are induced to produce and secrete at least one protein soluble in the culture medium having anti-HIV activity;
- (b) separating the cells from the culture medium containing the protein having anti-HIV activity;
- and
- (c) recovering the protein from the medium, wherein the protein is characterized by a molecular weight of about 7,000 to about 12,000 daltons as determined by gel filtration anaylsis.
A cellular protein having anti-HIV activity produced by
- (a) culturing CD4 positive T cells or myeloid cells in a tissue culture medium comprising PC6 to activate the cells;
- (b) incubating said tissue culture medium for a period of time sufficient to induce said cells to produce a protein having anti-HIV activity;
- (c) separating the cells from the culture medium containing the protein having anti-HIV activity;
- and
- (d) recovering the protein from the medium, by passing the medium containing the anti-HIV protein through a 30 kDa molecular sieve and retaining that portion of the medium which passed through the sieve, wherein the protein is characterized by a molecular weight of about 7,000 to about 12,000 daltons as determined by gel filtration analysis.
Dependencies are inferred from the claim text.