US 5,346,831 AGranted

Cytorich process system

AssigneeHoffman-La Roche Inc.

InventorsCharles L. Carrico, Jr., William A. Fox, James W. Geyer, Ernest A. Knesel, Jr.

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Abstract

A process for producing a monolayer of cytological material on an optically-clear, cationically-charged substrate, includes the steps of separating the cytological material by centrifugation over a density gradient, producing a packed pellet of the cytological material, mixing the pellet of the cytological material, withdrawing an aliquot of a predetermined volume of the material from the mixed pellet of the cytological material, depositing the aliquot and a predetermined volume of water into a sedimentation vessel, which is removably secured to the substrate, allowing the cytological material to settle onto the substrate under the force of gravity, and after settlement of the cytological material, removing the water from the sedimentation vessel.

Claims11

Dependent claims

A method for preparing a monolayer of a cytological material on a cationically-charged substrate, which comprises the steps of:

(a) separating the cytological material by centrifugation over a density gradient;
(b) producing a packed pellet of the cytological material;
(c) mixing the pellet of the cytological material;
(d) withdrawing an aliquot of a predetermined volume from the mixed pellet of the cytological material;
(e) depositing the aliquot in a predetermined volume of water into a sedimentation vessel having a base, which vessel is removable secured to the cationically-charged substrate;
(f) allowing the cytological material to settle onto the substrate under the force of gravity without flow of the aliquot and the water from the base of the sedimentation vessel;
(g) after settlement of the cytological material, removing the water from the sedimentation vessel.

4 more elements

Dependencies are inferred from the claim text.

Facts

Application: 08/112,001Filed 30 Aug 1993 · continuation in part
Priority: 29 Sep 1992
Granted: 13 Sep 1994
Term ends: Not estimatedThe source records don’t support an estimate.

Classification6 codes

G01N 1/2813
Preparing specimens for investigation including physical details of chemical methods covered elsewhere, e.g. G01N33/50, C12Q: producing thin…
G01N 2001/2846
Producing thin layers of samples on a substrate, e.g. smearing, spinning-on: cytocentrifuge method
Y10T 436/112499
Automated chemical analysis: with sample on test slide
Y10T 436/25
Chemistry: analytical and immunological testing: including sample preparation

Family

Serial953,036Parent of this continuation-in-part · filed 29 Sep 1992
US 5,346,831 AThis patent

From the US filing history. Foreign family members aren’t listed.

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