Glycerol dehydrogenase, process for its production and its use

Abstract

Pyrroquinoline quinone-dependent glycerol dehydrogenase is provided which requires no surfactant for its solubilization and stabilization. The glycerol dehydrogenase has the following properties: (a) catalyzing the following reaction: glycerol+electron acceptor.dbd.glyceraldehyde+reduced electron acceptor, (b) optimum pH: pH 8-9, (c) pH stability: stable at pH 7-11, (d) optimum temperature: 20.degree.-25.degree. C., (e) thermal stability: stable at a temperature of 30.degree. C. or less for 10 minutes at a pH of 7.0, (f) molecular weight: 70,000 by gel filtration and SDS polyacrylamide gel electrophoresis, (g) bound to pyrroloquinoline quinone as a prosthetic group, (h) soluble and stable without the need of an ionic or non-ionic surfactant.

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