Method for the conjugative transfer of mobilizable vectors for E. coli to gram-positive bacteria and vectors suitable for use in such a method
AssigneeDegussa AG
InventorsAndreas Schafer, Jorn Kalinowski, Alfred Puhler
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Abstract
The present invention relates to a method of effecting the conjugative transfer of a mobilizable vector from cells of an E. coli mobilizer strain to gram-positive bacterial cells. The invention also relates to vectors suitable for use in such a method.
Claims20
A method for the conjugative transfer of a mobilizable vector from cells of an E. coli mobilizer strain to Coryneform bacterial cells which comprising producing restriction-defective cells of said Coryneform bacteria and crossing said defective cells with cells of said E. coli strain carrying said vector under conditions such that transfer of said vector to said defective cells is effected.
A mobilizable, non-self-transferrable vector, comprising:
- i) a first DNA segment containing a replicon functional in E. coli;
- ii) a second DNA segment containing DNA sequences coding for mobilization (mob site) and transfer (oriT);
- and
- iii) a third DNA segment that recombines homologously in Coryneform bacteria or that contains a replicon functional in Coryneform bacteria.
A mobilizable, non-self-transferrable vector comprising:
- i) a first DNA segment containing a replicon functional in E. coli;
- ii) a second DNA segment containing DNA sequences coding for mobilization (mob site) and transfer (oriT);
- and
- iii) a Procaryotic transposon.
A vector pECM1, accession number DSM 4982.
A vector pECM3 having the restriction map shown in FIG. 2.
Dependencies are inferred from the claim text.