Capillary zone electrophoretic detection of biological thiols and their S-nitrosated and oxidized derivatives

A method for separating and detecting individual thiol compounds in a biological sample comprising the steps:

• (a) determining the elution time of known individual thiols to establish thiol standards:
• (b) subjecting said biological sample to capillary electrophoresis;
• and
• (c) comparing the elution times obtained for each component present in the sample, to the known elution times of the thiol standards prepared in step (a), to determine the identity of each individual thiol compound present in the sample.

A method for separating and detecting individual S-nitrosothiol compounds in a biological sample comprising the steps:

• (a) determining the elution time of known individual S-nitrosothiols to establish S-nitrosothiol standards;
• (b) subjecting said biological sample to capillary electrophoresis;
• and
• (c) comparing the elution times obtained for each component present in the sample to the known elution times of the S-nitrosothiol standards prepared in step (a), to determine the identity of each individual thiol compound present in the sample.

A method for separating and detecting oxidized derivatives of individual S-nitrosothiol compounds in a biological sample comprising the steps:

• (a) determining the elution time of known oxidized derivatives of individual thiol compounds to establish oxidized derivative standards;
• (b) subjecting said biological sample to capillary electrophoresis;
• and
• (c) comparing the elution times obtained for each compound present in the sample to the known elution times of the oxidized derivative standards prepared in step (a), to determine the identity of each individual oxidized thiol derivative present in the sample.