Proteins and the preparation thereof
AssigneeBASF SE
InventorsHans-Georg LeMaire, Heinz Hillen, Achim Moeller, Lothar Daum, Thomas Doerper, Thomas Subkowski
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Abstract
Novel proteins which have a molecular weight of about 42,000 daltons and have at the N terminus the amino acid sequence Xaa Thr Pro Tyr Ala Pro Glu Pro Gly Set Thr Cys Arg Leu Arg Glu where X is hydrogen, a phenylalanine residue (Phe) or the amino acid sequences Ala Phe, Val Ala Phe, Gln Val Ala Phe, Ala Gln Val Ala Phe, Pro Ala Gln Val Ala Phe or Leu Pro Ala Gln Val Ala Phe, and which are suitable for the treatment of diseases, are described.
Claims3
A purified and isolated TNF.alpha.-binding protein which has a molecular weight of about 42,000 daltons and has at the N terminus the amino acid sequence
- Xaa Thr Pro Tyr Ala Pro Glu Pro Gly Set Thr Cys Arg Leu Arg Glu
- where Xaa is hydrogen, a phenylalanine residue (Phe) or the amino acid sequences Ala Phe, Val Ala Phe, Gln Val Ala Phe, Ala Gln Val Ala Phe, Pro Ala Gln Val Ala Phe or Leu Pro Ala Gln Val Ala Phe.
A process for the preparation of a protein which has a molecular weight of about 42,000 daltons and has at the N terminus the amino acid sequence
- Xaa Thr Pro Tyr Ala Pro Glu Pro Gly Set Thr Cys Arg Leu Arg Glu
- where Xaa is hydrogen, a phenylalanine residue (Phe) or the amino acid sequences Ala Phe, Val Ala Phe, Gln Val Ala Phe, Ala Gln Val Ala Phe, Pro Ala Gln Val Ala Phe or Leu Pro Ala Gln Val Ala Phe, which comprises concentration of the urine of patients with fever and subsequent purification of the retentate obtained in this way by ion exchange and affinity chromatography.
Dependencies are inferred from the claim text.