Structural gene of membrane-bound alcohol dehydrogenase complex, plasmid containing the same and transformed acetic acid bacteria

A recombinant plasmid comprising

• (a) vector pUC 18 having an ampicillin resistant gene derived from E. Coli,
• (b) a cleaved DNA of pTA 5001 and
• (c) a structural gene of membrane-bound alcohol dehydrogenase complex shown by FIG. 1 and having a molecular size of about 7.0 kilo bases comprising a protein having a molecular weight of about 72,000 as determined by SDS polyacrylamide gel electrophoresis, shown by nucleotide sequence SEQ ID NO. 1 and a protein having a molecular weight of about 44,000 as determined by SDS polyacrylamide gel electrophoresis, shown by nucleotide sequence SEQ ID NO. 2, which are derived from Acetobacter altoacetigenes.