Human uterine tissue plasminogen activator produced by recombinant DNA
AssigneeGenzyme Corporation
InventorsCha-Mer Wei, Nancy Hsiung, Vermuri B. Reddy, Jeffrey F. Lemontt, William Dackowski, Richard Douglas, Edward S. Cole, Richard D. Purcell, Jr., David Tai-Yui Lau
Life of this patent
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Abstract
A replicable expression vector capable, in a transformed host mammallian cell, of expressing a DNA sequence encoding active human TPA which, when expressed in the host cell, yields active TPA which lacks one or more of the TPA-bound N-linked carbohydrate moieties of naturally occurring human TPA.
Claims21
A recombinant DNA molecule which encodes human tissue plasminogen activator (TPA) and which differs from a naturally occurring DNA molecule encoding naturally occurring human TPA in that, in place of at least one of the Asn codons in the three DNA sequences which encode an Asn-X-(Ser or Thr) signal for N-linked glycosylation in said naturally occurring human TPA corresponding to amino acids 114-116, 181-184, and 445-447 of FIG. 1A-C hereof, there is substituted a Gln codon.
Modified tissue plasminogen activators wherein the modification consists of amino acid substitutions for at least one residue in one, or more than one, of the three Asn-X-(Ser/Thr) glycosylation signals corresponding to amino acids 114-116 181-184, and 445-447 of FIG. 1A-C hereof.
Modified human tissue plasminogen activator (TPA) which differs from naturally occurring human TPA in that, in at least one of the three Asn-X-(Ser or Thr) signals for N-linked glycosylation in said naturally occurring human TPA corresponding to amino acids 114-116, 181-184, and 445-447 of FIG. 1A-C hereof, there is substituted a Gln for the Asn.
Dependencies are inferred from the claim text.