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US 5,344,771 AGranted
Plant thiosterases
AssigneeCalgene, Inc.
InventorsHuw M. Davies, Toni A. Voelker
Life of this patent
Estimating the term…
Abstract
This invention relates to plant thioesterases, means to identify such proteins, amino acid and nucleic acid sequences associated with such protein, methods to obtain, make and/or use such plant thioesterases. Also, by this invention, the existence of a heretofore unproven factor critical to the biosynthesis of medium-chain fatty acids in plants is demonstrated.
Claims6
A method of producing C12:0 fatty acids in a Brassica seed cell, said method comprising:
- growing a Brassica plant having integrated into its genome a DNA construct, said construct comprising in the 5' to 3' direction of transcription, a transcriptional regulatory region functional in said Brassica seed cell, a translational regulatory region functional in said Brassica seed cell, a plant transit peptide encoding sequence, a DNA sequence encoding an Umbellularia californica C12:0 preferring acyl-ACP thioesterase functional in said Brassica seed cell, and a transcriptional termination region functional in said Brassica seed cell.
Dependencies are inferred from the claim text.