Use of saturated, ring-containing compounds as clearing solvents in histologic procedures
OwnerGerald W. Camienerinventor
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Abstract
A method of using saturated organic ring-containing compounds as clearing solvents for biological materials is described. The clearing solvents are equally effective or superior to conventional clearing solvents but they lack the toxic and noxious characteristics of conventional clearing solvents. The clearing solvents are used to replace the alcohol and/or other dehydrants in fixed biological materials and to remove wax from wax-imbedded biological materials.
Claims11
A method of preparing biological material for microscopic examination, comprising contacting said biological material with a clearing solvent to remove alcohol and/or other dehydrants contained in the biological material or to remove wax contained in the biological material, wherein the clearing solvent consists essentially of from 5% to 100% by weight of a compound or combination of compounds having a higher flashpoint than xylene which is selected from the group consisting of unsubstituted and substituted saturated, organic ring-containing compounds, hydrogenated aromatic petroleum distillates, and combinations thereof.
Dependencies are inferred from the claim text.