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US 5,034,320 AGranted
Vinblastine synthesis
AssigneeAllelix Inc.
InventorTsuyoshi Endo
Life of this patent
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Abstract
The reaction of 3',4'-anhydrovinblastine with a Catharanthus roseus-derived protein fraction to form vinblastine is improved by conducting the reaction in the presence of a reducing agent such as the enzyme cofactor NADH. A cationic species such as manganous ion may also be added to the reaction. Vinblastine yields are enhanced.
Claims5
A method for enhancing vinblastine yield from a reaction in which 3', 4'-anhydrovinblastine and C. roseus-derived enzyme are reacted in a reaction medium to produce vinblastine, said method comprising the steps of:
- (a) adding to said reaction medium both NADH and a water soluble salt of a divalent cationic species selected from the group consisting of manganese, cobalt, copper and magnesium;
- (b) supplementing the reaction mixture formed in step (a) with NADH at a plurality of stages during the course of the reaction;
- (c) adding a reaction-halting amount of a base to the reaction medium prior to vinblastine recovery and at a time when vinblastine yields are maximal;
- and
- (d) then recovering vinblastine from the reaction medium.
2 more elements
Dependencies are inferred from the claim text.