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US 4,920,054 AGranted
Shuttle vectors from rhodococcus equi
AssigneeAllelix Inc.
InventorsMaya Kozlowski, Wayne Glasse-Davies
Life of this patent
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Abstract
Recombinant DNA plasmids capable of functioning as shuttle vectors in Rhodococcus equi, Corynebacterium, E. coli, B. subtilis, or S. aureus are disclosed. The recombinant plasmid contains an origin of replication which is functional in Rhodococcus and at least one of E. coli, Corynebacterium, S. aureus, and B. subtilis. Additionally, the plasmid contains a heritable, selectable marker.
Claims18
A plasmid which comprises:
- (a) a first minimal genetic region encoding an origin of plasmid replication functional in Rhodococcus;
- and
- (b) a second minimal genetic region, encoding an origin of plasmid replication functional in at least one selected from the group consisting of E. coli, Bacillus subtilis, Corynebacterium, and Staphylococcus aureus.
The plasmid of claim 10 wherein said transposable element is Tn917.
A host transformed with a plasmid wherein said plasmid comprises:
- (a) a first minimal genetic region, encoding an origin of plasmid replication functional in Rhodococcus;
- and
- (b) a second minimal genetic region, encoding an original of plasmid replication functional in at least one selected from the group consisting of E. coli, Bacillus subtilis, Corynebacterium, and Staphylococcus aureus.
Dependencies are inferred from the claim text.