In a plug-in connection for connecting tube and hose lines, comprising a male-part having thereon first means defining a clip-receiving, radially outwardly opening, locking groove, a female-part having second means thereon adapted to sealingly receive therein said male-part, said female-part having third means defining a radially inwardly opening cavity means thereon adapted to be radially aligned with said locking groove, a locking clip with legs, said legs of said locking clip being adapted to be received in both said cavity means in said female-part and said locking groove when radially aligned with each other, the improvement comprising each of said legs of said locking clip have fourth means defining an extension extending transversely to the longitudinal length of said leg, wherein said female-part has fifth means defining plural abutments in said cavity means adapted to engage and abut said extensions, said abutments being oriented adjacent the peripheral surface of said female-part, radially outside the region whereat said extensions are located when said locking clip serves to lock said male-part to said female-part, said locking clip being supported on said female-part for radial movement relative to said female-part, said locking clip being made of elastically yieldable material, said extensions being oriented, in a relaxed condition of said locking clip, adjacent one another spaced from said abutments, surface means on said female-part for effecting a spreading apart of said legs on said locking clip in response to said locking clip being moved radially of said female-part whereby a radial movement of said locking clip relative to said female-part will cause said extensions and said legs to spread apart to bring said legs wholly out of said locking groove to thereby release the lock holding said male-part in said female-part and to thereby facilitate a relative axial movement between said male-part and said female-part, said extensions eventually coming into abutting relation with said abutments during a continued radial movement to thereby prevent said locking clip from being removed from its connected relationship with said female-part and said legs thereof from being further spread apart.