Process for separating and producing chlorogenic acid
AssigneeErgo Forschungsgesellschaft mbH
InventorsReiner Kopsch, Claus F. Gosswein, Henning Lutz, Michael Ball, Peter Hubert
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Abstract
Chlorogenic acid can be separated and produced as free acid or as salts thereof from plant raw material extracts by means of gel permeation chromatography on cross-linked modified polysaccharides, especially on dextrans. To obtain the free acid, the aqueous extract must be adjusted to a pH in the range of about 2 to 2.8. By use of a column of sufficient length the isomeric 5-, 4- and 3-chlorogenic acid can also be separated by the process of the invention.
Claims8
A process for separation and production of 3-, 4-, and 5-chlorogenic acid by extraction of plant raw materials in water comprising passing a water extract from plant raw material containing chlorogenic acid through a chromatography column containing crosslinked dextran whereby the chlorogenic acid is separated from accompanying substances by gel permeation chromatography on said cross-linked dextran and eluting said chlorogenic acid with water as elution medium to obtain a purity of greater than 70.7% in commercial scale amounts.
A process for the quantitative separation and production of 3-, 4- and 5-chlorogenic acid by extraction of plant raw materials comprising passing a water extract from plant raw material containing chlorogenic acid having a pH that has been adjusted to a range of about 2.0 to about 2.8 through a chromatography column containing cross-linked dextran whereby the chlorogenic acid is separated from the accompanying substances by gel permeation chromatography on a cross-linked dextran and eluting said chlorogenic acid on an elution medium consisting essentially of water and recovering chlorogenic acid at a purity of greater than 70.7% in commercial scale amounts.
Dependencies are inferred from the claim text.