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AssigneeAmerican Biogenetic Sciences, Inc.
InventorsMalcolm J. Fraser, Elliot D. Rosen, Victoria A. Ploplis
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The present invention is directed to recombinant baculoviruses which encode fusion polyhedrin proteins capable of forming occlusion bodies containing foreign peptides. The recombinant baculoviruses of the invention are formed by insertion into or replacement of regions of the polyhedrin gene that are not essential for occlusion body formation, with foreign DNA fragments by recombinant DNA techniques. The recombinant occlusion bodies produced in accordance with the present invention have uses in vaccine formulations, immunoassays, immobilized enzyme reactions, as biological insecticides, and as expression vectors.
A recombinant occlusion body comprising repeating subunits in which each subunit comprises a polyhedrin fusion protein comprising a portion of the polyhedrin protein which participates in crystallization, fused to a foreign amino acid sequence.
A polyhedrin fusion protein which is capable of crystallizing with other polyhedrin proteins to form recombinant occlusion bodies, comprising:
A recombinant virus which directs the expression of polyhedrin fusion proteins that crystallize to form recombinant occlusion bodies, comprising:
A transfer vector encoding a polyhedrin fusion protein that crystallizes to form recombinant occlusion bodies comprising:
A recombinant vector, pBRX13, substantially as deposited with the NRRL and assigned accession number B18312.
Dependencies are inferred from the claim text.