Process for removing nucleic acids and purine bases from gelatin
AssigneesForte Fotokemial Idpar, Chinoin Gyogyszervegyeszeti Gyar
InventorsMiklos Szucs, Istvanne Palotas, Jozsef Szejtli, Eva Fenyves
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Abstract
The invention relates to a process for removing nucleic acids and purine bases from gelatine by (a) adding cyclodextrin bead polymer having a particle size of 20 to 600 .mu., a cyclodextrin content of 30 to 70% and a polyvinyl alcohol content of 0.3 to 0.5% to the gelatin solution in a weight ratio of 1:1 to 10:1 calculated on the amount of the gelatin then filtering off the cyclodextrin polymer or (b) passing the gelatin solution through a cyclodextrin bead polymer column, whereby the amount of the gelatin is 5 to 50% of the weight of the column charge, then eluting the nucleic acids and the purine bases from the cyclodextrin bead polymer.
Claims5
A process for removing nucleic acids and purine bases from gelatin comprising bringing into contact a gelatin solution and a cross-linked beta-cyclodextrin bead polymer having a particle size of about 20 to 600 microns, a beta-cyclodextrin content of about 30% to 70% by weight and a polyvinyl alcohol content of about 0.3% to 0.5% by weight;
- separating said cross-linked polymer and said gelatin solution to obtain a gelatin solution substantially free from nucleic acids and purine bases.
Dependencies are inferred from the claim text.