Device for performing qualitative enzyme immunoassays
AssigneeAllelix Inc.
InventorHoward M. Chandler
Life of this patent
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Abstract
The invention relates to a device and method for performing qualitative enzyme immunoassays. The device comprises at least one tube having antibody, antigen or hapten attached to an internal surface thereof. A first syringe is connectable to a first end of the tube or tubes and supplies a flow of test liquid therethrough. A second syringe having a two piston arrangement is connected to the tube so as to allow a first wash solution followed by an enzyme conjugate solution to be flowed therethrough. Finally a third syringe, also having a two piston configuration, is connected to the tube so as to allow a second wash solution followed by an enzyme substrate and metabolite indicator solution to be flowed therethrough.
Claims13
A device for performing an enzyme immunoassay, comprising:
- at least one assay tube having a first and a second end and having an antibody, antigen or hapten attached to an internal surface thereof;
- a first syringe having an outlet connectable to the first end of the assay tube for supplying a flow of a test liquid therethrough;
- a second syringe having an outlet connected to the first end of the assay tube for supplying a flow of a first wash solution followed immediately by a flow of an enzyme conjugate containing solution therethrough, said second syringe having a first piston and a second piston spaced from one another, the first piston being positioned to separate the first wash solution from the conjugate solution, the second piston having a plunger, and said syringe also having a wall with a groove therein connecting the syringe outlet with the conjugate solution only when the first piston is fully depressed;
- and
- a third syringe having an outlet connected to the first end of the assay tube for supplying a flow of a second wash solution followed immediately by a flow of solution containing an enzyme substrate and an enzyme metabolite indicator therethrough, said third syringe having a first piston and a second piston, spaced from one another, the first piston being positioned to separate the second wash solution from the substrate, the second piston having a plunger, said third syringe also having a wall with a groove therein connecting the syringe outlet with the substrate and indicator solution only when the first piston is fully depressed.
Dependencies are inferred from the claim text.