US 4,456,550 A · Vascular permeability factor

Abstract

A purified vascular permeability protein factor and method of manufacture, wherein the factor has the following characteristics: (a) in an aqueous solution (0.01 M Na.sub.3 PO.sub.4, pH 7) whose concentration of NaCl is varied linearly, the factor is eluted from a heparin-Sepharose chromatography column in a peak centered at 0.4 M NaCl; (b) in an aqueous solution of Na.sub.3 PO.sub.4 (pH 7.0) whose concentration is varied linearly, the factor is eluted from a hydroxylapatite column in a peak centered at 0.25 M Na.sub.3 PO.sub.4 ; (c) when subjected to SDS gel electrophoresis in a 7.5% polyacrylamide slab gel (0.375 M tris-HCl (pH 8.8), 0.1% SDS) at 35 milliamps and 4.degree. C., the factor is localized to a region corresponding to a molecular weight between 34,000 and 45,000 daltons.

Claims9

Dependent claims

A fast-acting purified protein factor which affects vascular permeability within 5 minutes of the time of injection and has little residual effect 20 minutes after injection and has the following characteristics:

(a) in an aqueous solution (0.01 M Na.sub.3 PO.sub.4, pH 7) whose concentration of NaCl is varied linearly, the factor is eluted from a heparin-Sepharose chromatography column in a peak centered at 0.4 M NaCl;
(b) in an aqueous solution of Na.sub.3 PO.sub.4 (pH 7.0) whose concentration is varied linearly, the factor is eluted from a hydroxylapatite column in a peak centered at 0.25 M Na.sub.3 PO.sub.4 ;
(c) when subjected to SDS gel electrophoresis in a 7.5% polyacrylamide slab gel (0.375 M tris-HCl (pH 8.8), 0.1% SDS) at 35 milliamps, 4.degree. C., the factor is localized to a region corresponding to a molecular weight between 34,000 and 45,000 daltons.

Dependencies are inferred from the claim text.

Vascular permeability factor

Life of this patent

Vascular permeability factor

Life of this patent

Abstract

Claims9