Method and agent for the stabilization of alkaline phosphatase
AssigneeMerck Patent GmbH
InventorsRolf Vormbrock, Roland Helger
Life of this patent
Estimating the term…
Abstract
Alkaline phosphatase is stabilized in buffer solutions. This is accomplished by adding a water soluble complex of zinc ions and a chelating agent to the buffer.
Claims9
In an aminoalcohol-containing buffer solution reagent for use in the determination of the alkaline phosphatase present in a test sample brought into contact with the buffer solution, the improvement wherein the aminoalcohol-containing buffer solution further comprises the products of the addition thereto of an amount of a water-soluble zinc chelate complex effective to stabilize the alkaline phosphatase when the buffer solution is contacted with the test sample.
A method for stabilizing the alkaline phosphatase in a buffered solution of the same containing an aminoalcohol, comprising adding to the buffered solution a stabilization effective amount of a preformed water soluble zinc chelate complex or of zinc ions and the chelating agent which make up the complex.
Dependencies are inferred from the claim text.