Process for determination of glycosylated hemoglobin and reagent therefor
AssigneePanchem Gesellschaft fur chemische Produkte mbH
InventorsCornelia Niederau, Hans Reinauer
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Abstract
An improved process for determining glycosylated hemoglobin wherein the contribution of unstable glucose-aldimine-hemoglobin compounds to the HbA.sub.1 value is substantially eliminated by reacting the hemolysate with a primary amine and a hydrazine compound and thereafter subjecting the hemolysate to chromatography. The process enables reliable evaluation of the HbA.sub.1 value as a long-term parameter in diabetic metabolism control.
Claims27
In a process for the determination of the glycosylated hemoglobin content of a blood sample comprising the hemolysis of erythrocytes, the chromatographic separation of hemoglobin fractions and their colorimetric measurement, the improvement wherein the contribution of unstable glucose-aldimine-hemoglobin compounds to the HbA.sub.1 value is substantially eliminated by reacting the hemolysate with a primary amine and a hydrazine compound and thereafter subjecting the hemolysate to chromatography.
In a process for the determination of the glycosylated hemoglobin content of a blood sample comprising the hemolysis of erythrocytes, the chromatographic separation of hemoglobin fractions and their colorimetric measurement, the improvement wherein the contribution of unstable glucose-aldimine-hemoglobin compounds to the HbA.sub.1 value is substantially eliminated by reacting the hemolysate with hydroxylamine, an alkylamine, a hydroxyalkylamine or an amino acid and a hydrazine compound and thereafter subjecting the hemolysate to chromatography.
Dependencies are inferred from the claim text.