Back to results
US 4,399,225 AGranted
Stop-flow analysis
AssigneeBifok AB
InventorsElo H. Hansen, Jaromir Ruzicka
Life of this patent
Estimating the term…
Loading patent…
Loading patent…
AssigneeBifok AB
InventorsElo H. Hansen, Jaromir Ruzicka
Estimating the term…
A continuous method of quantitatively determining slowly reacting compounds with the use of a single measuring cell, the sample being injected into a continuously flowing carrier solution which is already provided with, or simultaneously with the sample is provided with reagent to form a sample zone in the form of a reproduceable gradient. Said sample zone is led into a measuring cell whereupon the flow is stopped by means of shunting the carrier flow, by stopping the pump, or by means of a valve. The reaction is allowed to take place in the measuring cell while a magnitude, characteristic for the reaction, is registered. The reaction speed can be detected from the incline of the obtained response curve and the amount of the sought-after compounds is calculated. By means of setting the stop at a certain point in the concentration gradient curve and always selecting said point at the same time distance from the peak of the gradient curve during flow, a total reproduceability of the analysis is ensured.
A method for determining the reaction speed of a sample at selected sample concentrations within a sample mixture slug comprising the steps of:
In a method of analysis where a sample is introduced into a flowing carrier stream to form a sample mixture slug having a forward head portion, a rearward tail portion and a central portion, said sample dispersing to form reproducable concentration gradients in said head and tail portions in which the concentration of said sample varies, said sample undergoing a reaction while in said carrier stream, wherein the improvement comprises measuring the reaction speed of said sample at one or more selected sample concentrations within said concentration gradients by stopping the flow of said carrier stream and measuring the reaction of said sample at said selected sample concentrations for a period of time.
A method of determining the change in a changing characteristic of a sample at selected sample concentrations comprising the steps of:
Dependencies are inferred from the claim text.