Process for isolating microbiologically active material
AssigneeMassachusetts Institute of Technology
InventorJames C. Weaver
Life of this patent
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AssigneeMassachusetts Institute of Technology
InventorJames C. Weaver
Estimating the term…
Molecules or cells of materials exhibiting a microbiological activity, suppression or interaction of interest are isolated from a large population of similar molecules, cells or the like. The activity of the microbiologically active molecules or living matter of interest is measured by suspending the population in a liquid medium capable of forming a gel. The resultant suspension is formed into droplets which are caused to gel. The gel droplets (GMDs) are treated to effect desirable alteration of the microbiologically active material and the amount of metabolites or reaction products of the desired alteration within each of the gel droplets. Alternatively, incubation is carried out such that each GMD initially containing one cell contains many, a microcolony, which can be tested for desirable properties while retaining sufficient viable cells for further growth and harvesting for further tesing and/or production. The gel droplets are then separated based upon the measured or sensed characteristic, metabolite or reaction product.
The process of selecting molecules or cells having a desired microbiological activity of a sample of a given material comprising the steps of:
The process for selectively isolating gel microdroplets containing a cell or molecule having microbiological activity having a desired microbiological characteristic from a large population of microbiologically active cells or molecules lacking said desired characteristic which comprises forming a dilute suspension of said population in a liquid diluent capable of forming a gel upon subsequent treatment, said dilution being selected so that there is provided a high probability that each sub-sample produced from said suspension contains one or less microbiologically active molecule or cell, converting said suspension into gel droplets having a size between about 0.2 microns and about 1000 microns sensing the gel droplets containing the microbiologically active material having the desired characteristic and selectively separating said gel droplets containing microbiologically active material having the desired characteristic from said population.
Dependencies are inferred from the claim text.