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US 4,399,217 AGranted
Process and a device for the determination of serum lipoproteins
AssigneeLaboratoires Goella
InventorsLeif T. Holmquist, Lars A. Carlson
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AssigneeLaboratoires Goella
InventorsLeif T. Holmquist, Lars A. Carlson
Estimating the term…
The invention relates to a process for the determination of serum lipoproteins by an immuno-enzymatic method. The process of the invention consists in reacting a specific antibody of the apoprotein whose amount is to be measured, fixed on a support, with, on the one hand, the compound for coupling the corresponding lipoprotein with an enzyme and, on the other hand, the analyzed sample. The amount of enzyme fixed is then an inverse function of the apoprotein content of the sample. The support with the products which are fixed thereto are separated from the rest of the reagents and the activity of the enzyme of the support is measured which is compared with standard measurements. The process is useful for serum analyses, particularly for early detection of cardiovascular disease risks.
Process for the determination of serum apoproteins consisting in reacting, on the one hand, a given amount of a specific antibody of the apoprotein whose amount is to be determined, said antibody being fixed on an inert solid support, and, on the other hand, the serum sample to be analyzed and a given amount of an enzyme-lipoprotein coupling compound, corresponding to the apoprotein whose amount is to be determined, after completion of the antibody-lipoprotein reaction separating the support from the non-fixed reagents, and then measuring the enzymatic activity of the complex retained on the support, and wherein said coupling compound is formed by fixation of an enzyme on a fraction of serum lipoprotein containing several distinct apoproteins.
A kit assembly for determination of serum apoproteins consisting essentially of at least one vessel containing at least one immobilized specific antibody of a serum apoprotein and the corresponding lipoprotein(s)-enzyme.
Dependencies are inferred from the claim text.