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AssigneeTechnicon Instruments Corporation
InventorsClaude H. Moussebois, Pierre L. Masson, Jean-Pierre Vaerman, Joseph Limet, Cesar L. Cambiaso
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Immunoassays for antigens or haptens are effected using, instead of an immunoglobulin antibody, the F(ab').sub.2 fragments thereof. In this way, interference from endogenous RF and Clq in the fluid under assay is avoided without the necessity of pre-treating the fluid to remove or inactivate the RF or Clq. The assays may be of various types including latex agglutination and competitive binding assays. A reagent for such use comprising F(ab').sub.2 fragments insolubilized on a water-insoluble substrate, especially a particulate substrate, is described.
A method of immunoassay which comprises reacting an antigen in a fluid with insolubilized F(ab').sub.2 fragments of an immunoglobulin which is specific to said antigen, the reaction being effected in the substantial absence of the (whole) immunoglobulin and of F(c) fragments thereof.
A method of assaying a fluid by agglutination for an antigen therein, which comprises the steps of:
A reagent for use in immunoassay which comprises a suspension of finely divided particulate material having bound thereto the F(ab').sub.2 fragments of an immunoglobulin, the suspension being substantially free from the said whole immunoglobulin and F(c) fragments thereof.
A method of assaying a fluid by agglutination for an antigen therein, which comprises the steps of:
Dependencies are inferred from the claim text.