Forced precipitation method for preparing antigen/antibody particles
AssigneeResearch Corporation
InventorKarim E. Hechemy
Life of this patent
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Abstract
Process for the preparation of test reagents comprising antigens or antibodies adsorbed on a surface, for example, the surface of synthetic or natural polymer particles in which the test material to be adsorbed is dissolved in a solvent in contact with the adsorbing surface and precipitated by the addition of a liquid which is miscible with the solvent, but does not dissolve the test material.
Claims20
A process for the preparation of test reagents suitable for serological testing of mammalian sera for the presence of antigens or antibodies, said reagent comprising a test material of antigen or antibody adsorbed on the adsorbent surface of an adsorbent substrate which comprises preparing a solution containing dissolved test material in a solvent in the presence of an adsorbent substrate and adding to the solution an organic liquid precipitating reagent which is miscible with the solvent but does not dissolve the test material or the adsorbent substrate whereby the test material precipitates as an adsorbent on the surface of the adsorbent substrate.
A process for the preparation of test reagents for serological testing of mammalian sera for the presence of antigens or antibodies said reagent comprising a test material of antigen or antibody adsorbed on the adsorbent surface of an adsorbent substrate which comprises preparing in a container an aqueous solution said solution containing dissolved test material in the presence of the adsorbent substrate and adding to the solution a mixture of a water soluble alkali metal salt and a lower water soluble alcohol whereby the test material precipitates as an adsorbent on the adsorbent surface.
A process for the preparation of tests reagents suitable for serological testing of mammalian sera for the presence of a rickettsial infection which comprises preparing an aqueous solution containing a dissolved rickettsia antigen and suspended adsorbent particles and adding to the solution a mixture of a water soluble alkali metal salt and a lower water soluble alcohol whereby the antigen precipitates in the adsorbent surface.
A process for the preparation of test reagents suitable for serological testing of mammalian sera for the presence of rheumatoid arthritis antibodies which comprises preparing solution of Fraction II globulin containing a suspension of latex particles and adding to the solution a mixture of water soluble alkali metal salt and a water soluble alcohol whereby the Fraction II globulin precipitates on the adsorbent surface of the latex particles.
A process for the preparation of test reagents suitable for serological testing of mammalian sera for the presence of Escherichia coli antibodies which comprises preparing a solution of dissolved lipopolysaccharide antigens from Escherichia coli containing a suspension of latex particles and adding to the solution a mixture of a water soluble alkali metal salt and a water soluble alcohol whereby the antigen precipitates on the adsorbent surface of the latex particles.
A process for the preparation of test reagents suitable for serological testing of mammalian sera for the presence of trichinosis antibodies which comprises preparing a solution of dissolved trichinosis antigen containing a suspension of latex particles and adding to the solution a mixture of a water soluble alkali metal salt and a water soluble alcohol whereby the antigen precipitates on the adsorbent surface of the latex particles.
A process for the preparation of test reagents suitable for serological testing of mammalian sera for the presence of toxoplasma antibodies which comprises preparing a solution of dissolved toxoplasma antigen containing a suspension of latex particles and adding to the solution a mixture of a water soluble alkali metal salt and a water soluble alcohol whereby the antigen precipitates on the adsorbent surface of the latex particles.
A process for the preparation of test reagents suitable for serological testing of mammalian sera for the presence of chlamydial antibodies which comprises preparing a solution of dissolved chlamydial antigen containing a suspension of latex particles and adding to the solution a mixture of a water soluble alkali metal salt and a water soluble alcohol whereby the antigen precipitates on the adsorbent surface of the latex particles.
Dependencies are inferred from the claim text.