Production of blood coagulation factor VIII:C

A method for the preparation of a concentrate of Factor VIII:C from blood plasma which comprises

• (a) admixing said blood plasma or a concentrate thereof at pH of from about 7.0 to about 8.5 with from about 0.01% to about 0.1% by weight of water-insoluble, polyelectrolyte copolymer of ethylene and maleic anhydride cross-linked with from about 3 mole % to about 10 mole % of loweralkyliminobis(loweralkylamine) and containing from about 90 mole % to about 100 mole % of pendant diloweralkylaminoloweralkylimide functional groups, said admixing in the presence of exogenous heparin,
• (b) separating the supernatant from the resulting adsorbed plasma fraction,
• (c) admixing said supernatant at pH of from about 5.5 to about 6.5 with from about 1% to about 10% by weight of water-insoluble, polyelectrolyte copolymer of ethylene and maleic anhydride cross-linked with from about 3 mole % to about 10 mole % of loweralkyliminobis(loweralkylamine), containing from about 3 mole % to about 7 mole % of pendant diloweralkylaminoloweralkylimide functional groups, and further characterized in that substantially all the remaining free carboxyl or anhydride sites are blocked with alkoxyalkylamine, said admixing in the presence of exogenous heparin,
• (d) separating the resulting adsorbed plasma fraction from the supernatant and recovering therefrom a concentrate of Factor VIII:C by elution from the adsorbent, and
• (e) wherein said alkyl and alkoxy have from about one to about four carbon atoms and the exogenous heparin is present in a range of from about 0.01 to about two units per ml of plasma.