Novel erythropoietin product and method for the preparation thereof
AssigneesAjinomoto Co., Inc., Japan Chemical Research Co., Ltd.
InventorsKenji Takezawa, Hajima Hiratani
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AssigneesAjinomoto Co., Inc., Japan Chemical Research Co., Ltd.
InventorsKenji Takezawa, Hajima Hiratani
Estimating the term…
The invention provides a novel erythropoietin product prepared from the urine of healthy human but exhibiting no inhibitory effect against erythropoiesis as is the most disadvantageous problem in the product prepared from the urine of healthy human. The method for the preparation of such a product comprises adsorbing the crude erythropoietin product obtained from the urine of healthy human on to a weakly basic anion exchanger from a neutral or weakly acidic aqueous solution containing 0.1 to 0.2 mole per liter of an inorganic neutral salt such as sodium chloride and then eluting the thus adsorbed ingredients with an aqueous eluant solution containing from 0.5 to 0.7 mole per liter of an inorganic neutral salt.
A method for the preparation of an erythropoietin product having no inhibitory effect against erythropoiesis which comprises the steps of
An erythropoietin product having no inhibitory effect against erythropoiesis with a molecular weight in the range from 4,000 to about 13,000 and an isoelectric point in the range from 3.1 to 3.6.
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