Preparations of purified bacterial membranal proteoglycans
AssigneePierre Fabre S.A.
InventorsLucien D. d'Hinterland, Gerard Normier, Anne-Marie Pinel, Jacques Durand
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Abstract
This invention relates to a process for the preparation of purified bacterial membranal proteoglycans. The process comprises at least one step in which crude proteoglycans are treated in aqueous medium with a base or a hypobromite, followed by elimination of the excess reactant and the insoluble residue, the purified proteoglycans being present in aqueous solution. The thus obtained purified proteoglycans may be used as immunity adjuvants in vaccines without causing side-effects such as pyrogenic reactions.
Claims14
An immunity adjuvant for vaccines comprising purified water-soluble proteoglycans obtained by a process which comprises at least one step in which crude membranal proteoglycans of Klebsiella, Serratia or Escherichia are treated in an aqueous medium with a reactant selected from the group consisting of bases and hypobromites followed by removal of the excess reactant and the insoluble residue, said purified water-soluble proteoglycans having the following composition by weight:
- ______________________________________ hexoses 24-42% proteins 31-53% lipids 12-18% hexosamines 2-6% RNA <0.5% DNA <0.2% LPS <0.001% ______________________________________
Dependencies are inferred from the claim text.