Heparinase derived anticoagulants
AssigneeMassachusetts Institute of Technology
InventorsRobert S. Langer, Robert J. Linhardt, Charles L. Cooney, Gerald Fitzgerald, Arthur Grant
Life of this patent
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Abstract
There is disclosed a heparin product obtained by degradation of heparin with heparinase from Flavobacterium heparinum (ATCC 13125) or mutants thereof having activity to reduce the coagulation activity of factor X while not effecting the coagulation activity of thrombin.
Claims5
An anticoagulant composition effective to reduce the coagulant activity of factor X, while being ineffective to reduce the coagulant activity of thrombin said composition being produced by digesting heparin with a purified heparinase obtained from Flavobacterium heparinum (ATCC 13125) or mutants thereof and comprising degradation products from said digestion selected from the group consisting of a mixture of saccharides, having an average chain size of between about 2.5 and about 3.5, an average molecular weight of between about 700 and about 1320, a tetrasaccharide isolated from said mixture having a chain size of 4 and a molecular weight between about 100 and about 1500, a hexasaccharide isolated from said mixture having a chain size of 6 and a molecular weight between about 1700 and 2200 and an oligosaccharide composition isolated from said mixture having a chain size equal to or greater than 8 and having an average molecular weight between about 2200 and about 4000.
Dependencies are inferred from the claim text.