N-Sulfoalkylaniline derivatives
AssigneeDojindo Laboratories
InventorsMasanobu Maeda, Yuko Murao
Life of this patent
Estimating the term…
Abstract
N-sulfoalkylaniline derivatives in which at least one sulfoalkyl of 2 to 4 carbon atoms is attached to the nitrogen atom which are useful as dyestuff forming substances, a composition for determining the presence of peroxides using such compounds and the process for determination of peroxides using such compounds.
Claims9
A composition for determing the presence of peroxides, comprising a crystalline compound of the formula (I):
- ##STR5## wherein R.sup.1 is selected from the group consisting of hydrogen, lower alkyl and sulfoalkyl of 2-4 carbon atoms;
- R.sup.2 is sulfoalkyl of 2-4 carbon atoms;
- R.sup.3 is selected from the group consisting of hydrogen, hydroxy and lower alkyl and lower alkoxy;
- and
- R.sup.4 is selected from the group consisting of hydrogen, lower alkoxy and lower alkyl, or the alkali metal or alkaline earth metal salt thereof, and a color-developer substance that will react with hydrogen peroxide selected from the groups consisting of 4-aminoantipyrine and 3-methyl-benzothiazolinonehydrazone and a said compound to form a dyestuff in the presence of peroxidase or a transition metal.
A process for determination of peroxides, which comprises adding to a sample containing an unknown quantity of a material capable of liberating peroxide upon peroxidation, a crystalline compound of the formula (I):
- ##STR6## wherein R.sup.1 is selected from the group consisting of hydrogen, lower alkyl and sulfoalkyl of 2-4 carbon atoms;
- R.sup.2 is sulfoalkyl of 2-4 carbon atoms;
- R.sup.3 is selected from the group consisting of hydrogen, hydroxy and lower alkyl and lower alkoxy;
- and
- R.sup.4 is selected from the group consisting of hydrogen, lower alkoxy and lower alkyl, or the alkali metal or alkaline earth metal salt thereof, a color-developer substance that will react in an oxidative-condensation with said compound to form a dyestuff and a peroxidase or a transition metal, and measuring the absorbency of the solution at maximum absorption.
Dependencies are inferred from the claim text.