Process for preparing lipoid-biologically active particles
AssigneeHuman Oltoanyagtermelo es Kutato Intezet
InventorsZoltan Riedl, Laszlo Sztankov, Istvan Horvath
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Abstract
Adjuvant particles are prepared from lipoid-soluble substances, and compositions comprising the particles and biologically active substances adsorbed thereon are provided. The adjuvant systems are capable of binding a wide variety of biologically active substances, and can be used in vivo and in vitro as well.
Claims5
Process for the preparation of particles from lipoid-soluble substances and compositions containing said particles and biologically active substances bound to them, which comprises admixing an egg lecithin and cholesterol dissolved in absolute alcohol, with
- (a) a biologically active substance taken up in an aqueous electrolyte having a pH of 2 to 10, in a volume ratio of 1:1000-9:1, stirring the reaction mixture for at least one minute, allowing it to stand for at most 7 days and separating the particles precipitated from the solution;
- or
- (b) an aqueous electrolyte having a pH of 2 to 10, in a volume ratio of 1:1000 to 9:1, and isolating the precipitated particles
- (c) an aqueous electrolyte solution in a volume ratio of 1:1000 to 9:1, isolating the precipitated particles, adding them to a biologically active substance taken up in an aqueous electrolyte solution having a pH of 2 to 10, stirring the solution obtained for at least two minutes, allowing to stand for at most 7 days and isolating the particles formed, or
- (d) adding to the particles prepared according to variant (b) a biologically active substance taken up in an aqueous electrolyte solution having a pH of 2 to 10 and forming a suspension.
Dependencies are inferred from the claim text.