US 4,396,601 A · Gene transfer in intact mammals

Abstract

Methods and compositions are provided for gene transfer to intact mammals with expression of the exogenous genetic material in the host. Mammalian host cells which are regenerative, normally highly proliferative or subject to induced proliferation, are transformed or modified in vitro with DNA capable of replication and expression in the host cell, wherein the DNA becomes incorporated into the cell. The modified cells are found to regenerate in the host with expression of the introduced DNA. Particularly, mammalian cells were modified with genes providing for overproduction of a particular enzyme. The modified cells were reintroduced in the host under conditions providing for selective advantage of the modified cells.

Claims15

Dependent claims

A method for introducing into a mammalian host exogenous genetic capability capable of expression in said host, which comprises:

isolating cells from a mammal or a syngeneic equivalent, where cells are from a regenerative body member to provide parent cells;
combining said parent cells with DNA, including at least one gene capable of providing a selective advantage over the parent cells, when said parent cells are subjected to mitotic inhibition, under conditions where said DNA is introduced into said parent cells by other than cell-cell fusion to provide modified cells;
introducing said modified cells into said host, wherein said modified cells return to said body member of said parent cells;
and
administering to said host said mitotic inhibitor to provide a selective advantage for said modified cells over said parent cells, whereby said modified cells regenerate in said host.

2 more elements

Dependencies are inferred from the claim text.

Gene transfer in intact mammals

Life of this patent

Gene transfer in intact mammals

Life of this patent

Abstract

Claims15