Apparatus for reflectance measurement of fluorescent radiation and composite useful therein

A composite useful for the separation of radiation of one wavelength from radiation of another different wavelength, comprising two components in a finely divided state dispersed in a vehicle binder which is substantially nonabsorptive of radiation of either of said wavelengths, one of said components being transmissive of radiation of said one wavelength and absorptive of radiation of said other wavelength, and the other component being reflective of radiation of both of said wavelengths.

Apparatus useful in reflectance measurement of fluorescent radiation emitted by an analyte during transition thereof from an excited state to a ground state, comprising an integrating sphere having an opening therein;

• a cuvette within said sphere adjacent said opening;
• means for directing incident radiation from an external source through said opening toward and through said cuvette;
• and a focusing mirror in said sphere on the side of the cuvette opposite said opening positioned to reflect back through the cuvette and towards said opening incident radiation which has passed through the cuvette, the inner surface of the integrating sphere comprising a composite of a first component transmissive of fluorescent radiation and absorptive of incident radiation, and a second component reflective of both fluorescent and incident radiation.

Apparatus useful in reflectance measurement of fluorescent radiation emitted by an analyte during transition thereof from an excited state to a ground state, comprising an integrating sphere within which an analyte can be subjected to incident radiation effective to raise such analyte to an excited state, the inner surface of said integrating sphere comprising a composite of two components, one of which is transmissive of fluorescent radiation and is absorptive of incident radiation, and the other of which is reflective of both incident and fluorescent radiation.