Method for purification of uricase
AssigneeMiles Laboratories, Inc.
InventorsDavid A. Brock, Surendra K. Gupta
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Abstract
A method is provided for purifying uricase by decreasing the amount of active catalase present. The method involves adjusting the pH of a catalase-containing uricase preparation to a pH in the range of about 11 to 13 to inactivate the catalase and recovering a uricase preparation substantially free of active catalase.
Claims7
A method for purifying uricase by decreasing the amount of active catalase present which comprises adjusting the pH of a catalase-containing uricase preparation to a pH in the range of about 11 to 13 to inactivate said catalase, and recovering therefrom a uricase preparation substantially free of active catalase.
A method for purifying uricase by decreasing the amount of active catalase present which comprises the steps of cultivating uricase-producing microorganism cells to produce uricase and catalase, adjusting the pH of the cells to a pH in the range of about 11 to 13 to inactivate said catalase, centrifuging said cells, disrupting said cells and recovering therefrom a uricase preparation substantially free of active catalase.
A method for purifying uricase by decreasing the amount of active catalase present which comprises the steps of cultivating uricase-producing microorganism cells to produce uricase and catalase, disrupting the cells, adjusting the pH of the cells to a pH in the range of about 11 to 13 to inactivate said catalase, and recovering therefrom a uricase preparation substantially free of active catalase.
Dependencies are inferred from the claim text.