Production of high-pyruvate xanthan gum on synthetic medium
AssigneeThe United States of America as represented by the Secretary of Agriculture
InventorsMartin C. Cadmus, Clarence A. Knutson, Jr.
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Abstract
A high-pyruvate xanthan gum, substantially free of insolubles and undesirable coloration, is produced by fermentation of Xanthomonas campestris on a carbohydrate-containing nutrient medium having (NH.sub.4).sub.2 HPO.sub.4 at a level of at least 0.15% as the primary nitrogen source, and having a total phosphate level of at least about 0.25%.
Claims3
In a process for producing polysaccharides by fermentation of a carbohydrate-containing nutrient medium with the bacterium Xanthomonas campestris, wherein said bacterium is cultivated on said medium under conditions suitable for its growth, the improvement comprising:
- a. providing (NH.sub.4).sub.2 HPO.sub.4 as the primary nitrogen source in said nutritent medium at a level of at least 0.15 g./100 ml. of medium;
- b. providing a total phosphate level in said nutrient medium of at least 0.25 g./100 ml. of medium;
- and
- c. recovering from said fermentation medium a polysaccharide isolated from residual solids in said medium wherein said polysaccharide has a pyruvic acid content of at least 3.3% by weight.
Dependencies are inferred from the claim text.