Sampling valve useful in liquid chromatography

Method of feeding samples in liquid chromatography, said method comprises the steps of:

• providing a sample into a dosing loop by means of an injection needle through a sample inlet valve said valve having stationary and movable members in a first position of said valve,
• changing said sample valve to a second position, thereby connecting, in series, a transport liquid source, said dosing loop and a separating column, and
• wherein said providing step includes:
• sucking an air volume into said injection needle prior to sucking-in said sample;
• inserting said injection needle, through a bore in said movable valve member into a dosing bore of said stationary valve member,
• retracting said injection needle after the sample has been introduced and prior to said changing-over of said sample valve.