Assay method and reagent for the determination of chloride
AssigneeAmerican Monitor Corporation
InventorThomas W. Stephens
Life of this patent
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Abstract
A colorimetric assay method and reagent composition for the direct, quantitative determination of chloride in fluids which may be performed directly on non-deproteinized samples and which utilizes a reagent comprised of 2,4,6-tripyridyl-s-triazine, mercuric ions, ferrous ions, and a sulfate salt in a sulfate or sulfonate buffer at a selected pH in the range of about 1.5 to 3.0.
Claims8
A reagent composition for the determination of chloride in a protein-containing fluid, comprised of:
- (a) 2,4,6-tripyridyl-s-triazine;
- (b) mercuric ions;
- (c) ferrous ions;
- (d) at least one salt selected from the group consisting of sodium sulfate, manganese sulfate, magnesium sulfate, and cupric sulfate;
- and
- (e) a buffer selected from the group consisting of sulfuric acid and toluene sulfonate.
A method for the determination of chloride in a protein-containing fluid, comprising the steps of:
- (a) combining a sample of said fluid with a reagent to form a solution, said reagent comprising:
- (i) 2,4,6-tripyridyl-s-triazine,
- (ii) mercuric ions,
- (iii) ferrous ions,
- (iv) at least one salt selected from the group consisting of sodium sulfate, manganese sulfate, magnesium sulfate, and cupric sulfate;
- and
- (v) a buffer selected from the group consisting of sulfuric acid and toluene sulfonate;
- (b) measuring the absorbance of said solution;
- and
- (c) comparing the absorbance of said solution with the absorbance obtained on solutions containing known amounts of chloride.
A test kit for the determination of chloride in protein-containing fluid samples, containing a reagent which comprises:
- (a) 2,4,6-tripyridyl-s-triazine;
- (b) mercuric ions;
- (c) ferrous ions;
- (d) at least one salt selected from the group consisting of sodium sulfate, manganese sulfate, magnesium sulfate, and cupric sulfate;
- and
- (e) a buffer selected from the group consisting of sulfuric acid and toluene sulfonate.
Dependencies are inferred from the claim text.