Human hepatoma derived cell line, process for preparation thereof, and uses therefor
AssigneeThe Wistar Institute of Anatomy and Biology
InventorsBarbara B. Knowles, David P. Aden
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Abstract
Human hepatoma cell lines, useful for metabolic studies such as screening potential carcinogens and mutagens, for cultivation of viruses, and for preparation of vaccines is obtained by culturing human hepatocarcinoma or hepatoblastoma on lethally irradiated cell feeder layers in the presence of a culture medium.
Claims9
A process for assessing the metabolic conversion of chemicals and drugs considered to be potential carcinogens and mutagens, which comprises:
- (a) maintaining a culture of a human hepatic cell line selected from the group consisting of the cell lines designated Hep 3B and Hep G2 in a nutrient medium,
- (b) exposing said cell line to the chemical or drug to be tested,
- (c) analyzing said culture for the presence of metabolites of said chemical or drug, and
- (d) introducing said metabolites to cultures of other mammalian cells to determine their mutagenic capabilities.
A process for isolation of hepatitis B virus surface antigen for use as a vaccine which comprises:
- (a) maintaining a human hepatic cell line, designated Hep 3B, which contains the hepatitis B virus genome in nutrient culture medium,
- (b) recovering the supernatant fluid from said culture, and
- (c) purifying the hepatitis B virus surface antigen in said supernatant fluid for use as a vaccine.
A process for isolation of human plasma proteins which comprises:
- (a) maintaining a human hepatic cell line which synthesizes plasma proteins selected from the group consisting of the cell lines designated Hep 3B and Hep G2 in a nutrient culture medium,
- (b) recovering the supernatant fluid from said culture, and
- (c) separating plasma proteins from said supernatant fluid, and purifying said plasma proteins.
The cell line having the identifying characteristics of Hep 3B.
The cell line having the identifying characteristics of Hep G2.
Dependencies are inferred from the claim text.