Method for producing citric acids
AssigneeTakeda Chemical Industries, Ltd.
InventorsTakeshi Tabuchi, Matazo Abe
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Abstract
Citric acids are produced by culturing a citric acids-accumulating and hydrocarbon-assimilating strain of a yeast of the genus Candida in an aqueous medium containing, as main carbon source, at least one normal paraffin with from 9 to 20 carbon atoms, inclusive, in the molecule, at a specific pH, and recovering accumulated citric acids from the culture broth.
Claims34
A method for producing (+)-isocitric acid which comprises inoculating a (+)-isocitric acid-accumulating and hydrocarbon-assimilating strain of a yeast belonging to the genus Candida in an aqueous culture medium containing at least one normal paraffin containing 9 to 20 carbon atoms in the molecule as the main carbon source;
- incubating the culture at a pH of from about 4 to about 7.5 until (+)-isocitric acid is substantially accumulated in the culture broth;
- and separating the so-accumulated (+)-isocitric acid therefrom.
A method for producing citric acid and (+)-isocitric acid, which comprises inoculating a citric and (+)-isocitric acids-accumulating and hydrocarbon-assimilating strain of a yeast belonging to the genus Candida, in an aqueous culture medium containing at least one normal paraffin containing 9 to 20 carbon atoms in the molecule as a main carbon source;
- incubating the culture at a pH of from about 4 to about 7.5 until citric acid and (+)-isocitric acid are substantially accumulated in the culture medium, and separating the so-accumulated citric acid and (+)-isocitric acid therefrom.
A method for producing (+)-isocitric acid, which comprises inoculating a (+)-isocitric acid-accumulating and hydrocarbon-assimilating strain of a yeast belonging to the genus Candida, in an aqueous culture medium containing at least one normal paraffin containing 9 to 20 carbon atoms in the molecule as a main carbon source;
- incubating the culture under conditions of pH and temperature such that (+)-isocitric acid is accumulated, for a time until (+)-isocitric acid is substantially accumulated in the culture medium, and separating the so-accumulated (+)-isocitric acid form the culture medium.
Dependencies are inferred from the claim text.