Method for producing L-valine by fermentation
AssigneeAjinomoto Co., Inc.
InventorsHiroshi Matsui, Takayasu Tsuchida, Shigeru Nakamori
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Abstract
An L-valine-producing microorganism which is constructed by incorporation into a host strain of the genus Escherichia of a hybrid plasmid having inserted therein a DNA fragment with genetic information related to L-valine production which is derived from a donor strain of the genus Escherichia which is resistant to a valine analogue, is useful for the production of high levels of L-valine by fermentation.
Claims11
A method of producing L-valine by fermentation which comprises culturing in a culture medium an L-valine-producing microorganism selected from the group consisting of Escherichia coli NRRL B-12287 and Escherichia coli NRRL B-12288 which is obtained by incorporation into a recipient strain of the genus Escherichia, of a hybrid plasmid having inserted therein a DNA fragment with genetic information related to L-valine production which fragment is derived from a donor strain of the genus Escherichia which is resistant to a valine analogue, and recovering the L-valine accumulated in the culture medium.
A biologically pure culture of the microorganism Escherichia, having the identifying characteristics of NRRL B-12287, said culture being capable of producing L-valine in recoverable quantity upon fermentation in an aqueous medium containing assimilable nutrients.
A biologically pure culture of the microorganism Escherichia, having the identifying characteristics of NRRL B-12288, said culture being capable of producing L-valine in recoverable quantity upon fermentation in an aqueous medium containing assimilable nutrients.
Dependencies are inferred from the claim text.