Method for the purification of collagens
AssigneeLa Jolla Cancer Research Foundation
InventorsEva S. Engvall, Erkki I. Ruoslahti
Life of this patent
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Abstract
Collagen affinity matrices are prepared, useful for separating collagenous substances from other high molecular weight proteinaceous substances. Fibronectin is fragmentated chemically or by enzymatic digestion, etc. Fragments of fibronectin, which retain the collagen-binding site but are severed from other binding sites of intact fibronectin, are selected and coupled to solid support material.
Claims10
Affinity matrix material for separating collagen from other high molecular weight proteinaceous substances comprising:
- solid chromatographic support material;
- and
- at least one fragment of fibronectin which retains thereupon collagen binding sites, said fragment having been severed from other binding sites of intact fibronectin, said fragment being coupled with said solid chromatographic support material leaving collagen binding sites thereof exposed to selectively bind collagenous material when used chromatographically without binding interfering high molecular weight non-collagenous proteinaceous substances.
In the chromatographic method for separating proteinaceous substances from one another, in which a mixture of such substances is passed by way of a solution over a chromatographic material, the improvement comprising separating collagen from other proteinaceous sustances by passing the proteinaceous material containing solution over an affinity matrix material comprising:
- solid chromatographic support material;
- and
- at least one fragment of fibronectin which retains thereupon collagen binding sites, said fragment having been severed from other binding sites of intact fibronectin, said fragment being coupled with said solid chromatographic support material leaving callogen binding sites thereof exposed to selectively bind collagenous material when used chromatographically without binding interfering high molecular weight non-collagenous proteinaceous substances.
Dependencies are inferred from the claim text.