Method of preparing seeding material for production of citric acid
OwnerRoman Y. Karklin, Alma A. Rumba +1inventors
InventorsRoman Y. Karklin, Alma A. Rumba, Via K. Azanda
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Abstract
Disclosed is a method of preparing a seeding material for the production of citric acid by cultivating spores of a fungus on a nutrient medium containing carbon and nitrogen sources and mineral salts and by separating spores from the nutrient medium. The fungus used is the strain Aspergillus niger R-3 selected from the strain Aspergillus niger -119 by the stepwise action on it of ethyleneimine, N-nitrosomethylurea and ultraviolet radiation.
Claims3
A method of preparing a seeding material for the production of citric acid comprising cultivating the fungus Aspergillus niger R-3 strain TsMPMF 132 on a nutrient medium containing a carbon source selected from the group consisting of malt extract and beer must, a nitrogen source, and at least one mineral salt to form spores and separating said spores in an amount in excess of 1.3 g/dm from the nutrient medium.
Dependencies are inferred from the claim text.