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OwnerJury M. Lopukhin, Rem V. Petrov +7inventors
InventorsJury M. Lopukhin, Rem V. Petrov, Vitaly Y. Arion, Jury N. Breusov, Tatyana V. Gladysheva, Irina V. Sanina, Vasily V. Lebedev, Serafima S. Kirzon, Evgeny F. Ivanushkin
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The present invention relates to immunopharmacology and, more specifically, to a preparation controlling the T-system of immunity and to a method for producing the same. The preparation controlling the T-system of immunity is characterized by its content as the active principle, peptides with a molecular mass of from 1,500 to 6,000 Dalton having absorption maximum in UV-light at 280 and 275 nm and electrophoretic mobility in a polyacrylamide gel relative to bromophenol blue: 0.062-0.102; 0.156-0.236; 0.354-0.374; 0.382-0.422; 0.432-0.472; 0.485-0.545; 0.850-0.930. The method for producing the preparation according to the present invention comprises homogenization of a tissue of thymus gland in a solution of sodium chloride; the homogenizate is kept for 12-16 hours at a temperature of from 2.degree. to 6.degree. C., the residue is removed, thermolabile proteins are removed from the solution, peptides and proteins are precipitated and then dissolved and peptides are salted-out from the solution successively in three stages, the residue of peptides is removed, ultrafiltered through a membrane with a rated retention limit relative to globular proteins of from 12,000 to 30,000, the ultrafiltrate is gel-chromatographed to isolate peptides with a molecular mass of from 1,500 to 6,000 Dalton, desalted and lyophilized. This preparation is used for the treatment of primary and secondary immunodeficient states, diseases caused by malignant neoplasms, psoriasis and other pathological states.
An active principle effective for control of the T-system of immunity, said active principle being obtained by removing thermolabile proteins from a solution of homogenized thymus gland tissue in a sodium chloride solution, precipitating proteins and peptides from the solution, dissolving the same in a solvent therefor, salting out the peptides from the solution, dissolving the peptides, subjecting the resulting solution to ultrafiltration, and subjecting the resulting ultrafiltrate to gel chromatography, and said active principle consisting of polypeptides with a molecular mass of from 1,500 to 6,000 Dalton, having the maximum of absorption in UV-light as 208 and 275 nm and the electrophoretic mobility in a polyacrylamide gel relative to bromophenol blue of:
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