Back to results
US 4,089,747 AGranted
Compositions for the detection of hydrogen peroxide
AssigneeEastman Kodak Company
InventorBarbara Jungfleisch Bruschi
Life of this patent
Estimating the term…
Loading patent…
Loading patent…
AssigneeEastman Kodak Company
InventorBarbara Jungfleisch Bruschi
Estimating the term…
There are disclosed buffered compositions for detecting either hydrogen peroxide or a substance having peroxidative activity by the catalytic oxidation of a dye-providing material by hydrogen peroxide in the presence of the material having peroxidative activity, which compositions comprise (A) hydrogen peroxide or substance having peroxidative activity, as appropriate, and (B) as the dye-providing material, either (I) a mixture of (a) either a sulfonyl hydrazone of the formula ##STR1## or a sulfonyl hydrazone precursor of the formula ##STR2## wherein R = alkyl R' = hydrogen, aryl, alkoxy, halogen or alkyl R" = aryl or alkyl Z = atoms necessary to complete a heterocyclic ring of 5-6 atoms in the ring nucleus M.crclbar. = an anion; and (b) a coupler or (II) a triarylimidazole of the formula ##STR3## wherein R.sup.1, R.sup.2 and R.sup.3 are each an organic group such that at least one of R.sup.1, R.sup.2 and R.sup.3 is an ortho or para hydroxy substituted aryl group of up to 18 carbon atoms; the other two R.sup.1, R.sup.2 and R.sup.3 being chosen such that the oxidation potential of the imidazole lies between -70 mV to +110 mV measured by cyclic voltometry against a standard calomel electrode using a carbon based electrode. The method of utilizing such compositions and multilayer elements incorporating the same for the analysis of fluids suspected of containing hydrogen peroxide, substances having peroxidative activity or other analytes which produce hydrogen peroxide in their analysis are also described.
In a composition for the determination of hydrogen peroxide in aqueous solution, said composition comprising a substance having peroxidative activity and a dye-providing material which undergoes a detectable change upon contact with hydrogen peroxide and the substance having peroxidative activity, the improvement wherein the dye-providing material comprises:
In a composition for the detection of uric acid in aqueous solution, said composition comprising uricase, buffer, substance having peroxidative activity and a dye-providing material which undergoes a detectable change upon contact with hydrogen peroxide, in the presence of the substance having peroxidative activity, said hydrogen peroxide produced by the action of uricase of uric acid, the improvement wherein the dye-providing material comprises:
A composition for the assay of aqueous liquid for a predetermined analyte comprising (a) an enzyme which effects the production of hydrogen peroxide upon contact with said analyte and (b) a hydrogen peroxide detection composition comprising
In a method for assaying an aqueous liquid for hydrogen peroxide content comprising contacting a sample of the aqueous liquid with a buffered composition comprising a substance having peroxidative activity and a dye-providing material which is oxidized in the presence of hydrogen peroxide and said substance having peroxidative activity to provide a dye, the improvement comprising using as the dye-providing material ##STR46## a triarylimidazole of the formula ##STR47## wherein R.sup.1, R.sup.2, R.sup.3 are each an organic group such that at least one of R.sup.1, R.sup.2, and R.sup.3 is an ortho or para hydroxy substituted aryl group of up to 18 carbon atoms;
An element for the detection of uric acid in aqueous liquid, the element comprising a non-fibrous, isotropically porous spreading layer and a reagent layer and containing (a) uricase and (b) a hydrogen peroxide detection composition comprising
Dependencies are inferred from the claim text.