Process for preparation of cellulase
AssigneeAmano Pharmaceutical Co., Ltd.
InventorsTakaichi Ohya, Nobumasa Yokoi, Tamio Mase
Life of this patent
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Abstract
Aeromonas sp. No. 212 is cultured to allow it to produce a novel cellulase 212 and/or novel hemicellulase in a culture medium, and the so produced cellulase 212 and/or hemicellulase 333 is separated from the culture medium. The so separated novel cellulase 212 and/or novel hemicellulase 333 is used as a cellulosic material-decomposing agent in purifying tanks, plants for treatment of industrial waste waters, excrement treatment plants, drainage disposal treatment plants and the like.
Claims6
A process for preparing cellulase 212 comprising:
- aerobically culturing Aeromonas sp. No. 212, FERM-P No. 2306, ATCC No. 31085, in a culture medium containing a nitrogen source, a carbon source and a nutrient while maintaining the pH of the culture medium at 6-10 until cellulase is produced and accumulated therein;
- and
- separating and recovering the cellulase produced and accumulated in the culture medium during the culturing step.
A process for preparing hemicellulase 333 comprising:
- aerobically culturing Aeromonas sp. No. 212, FERM-P No. 2306, ATCC No. 31085, in culture medium containing a nitrogen source, a carbon source and a nutrient while maintaining the pH of the culture medium at 6-10 until hemicellulase is produced and accumulated therein;
- and
- separating and recovering the hemicellulase produced and accumulated in the culture medium during the culturing step.
A process for preparing both cellulase 212 and hemicellulase 333, comprising:
- aerobically culturing Aeromonas sp. No. 212, FERM-P No. 2306, ATCC No. 31085, in a culture medium containing a nitrogen source, a carbon source and a nutrient while maintaining the pH of the culture medium at 6-10 until both cellulase and hemicellulase are produced and accumulated therein;
- and
- recovering the cellulase and hemicellulase produced and accumulated in the culture medium during the culturing step.
Dependencies are inferred from the claim text.