Method for manufacture of .alpha.-galactosidase by microorganism
AssigneeHokkaido Sugar Co., Ltd.
InventorsShigeyoshi Narita, Hirosuke Naganishi, Akiyoshi Yokouchi, Ichiro Kagaya
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Abstract
When novel mold strains belonging to genus Absidia are cultured in an inducer-containing medium of the type ordinarily used for the culture of .alpha.-galactosidase producing molds, notably high .alpha.-galactosidase activity is produced and absolutely no invertase activity is produced in the mycelia. By treating beet molasses or beet juice with these mycelia, the yield of sucrose can easily be enhanced, since raffinose which is a substance impeding the crystallization of sucrose is effectively decomposed and sucrose is allowed to remain intact.
Claims6
A method for the manufacture of .alpha.-galactosidase, which comprises culturing a mold Absidia griseola (ATCC No. 20430) or Absidia griseola var. iguchii (ATCC No. 20431) in a culture medium containing substances inductive of .alpha.-galactosidase under conditions permitting the formation of .alpha.-galactosidase, and recovering the .alpha.-galactosidase.
A method of reducing the raffinose content of beet juice or beet molasses which comprises adding to said juice or molasses mycelia of the mold Absidia griseola (ATCC No. 20430) or Absidia griseola var. iguchii (ATCC No. 20431) cultured in a culture medium containing a substance inductive of .alpha.-galactosidase under conditions permitting the formation of .alpha.-galactosidase or the .alpha.-galactosidase prepared from said mycelial matter, and keeping the resultant mixture under conditions of enzymatic activity for said .alpha.-galactosidase until a portion of said raffinose has been decomposed.
Dependencies are inferred from the claim text.