High pressure fluid handling device for liquid chromatography

A high-pressure fluid handling device, comprising:

• an inlet configured to receive a liquid sample;
• a housing including:
• a first component having a first interface surface and a channel extending through the first interface surface, and
• a second component having a second interface surface, wherein the first and second components are securable to one another with the first and second interface surfaces in facing relationship with one another to form an interface therebetween;
• a tube positioned within the channel of the first component, the tube defining a lumen with a substantially circular cross-section;
• a stationary phase medium disposed within the lumen of the tube, wherein the stationary phase medium is suitable to chromatographically separate compounds present in the liquid sample;
• and
• an outlet port fluidically coupled to the inlet port via the tube.

A method of forming a high-pressure fluid handling device, comprising:

• providing a first component having a first interface surface;
• forming a channel into the first interface surface of the first component,
• the channel fluidically connecting an inlet opening of the first component to an outlet opening of the first component;
• positioning a tube within the channel, the tube including a lumen having a substantially circular cross-section with a diameter of less than or equal to 1000 μm;
• securing a second component having a second interface surface to the first component;
• positioning a filter at one of the inlet and outlet openings
• packing the lumen with stationary phase medium with a packing pressure of at least 5,000 psi, wherein the stationary phase medium is suitable to chromatographically separate compounds present in a liquid sample, and wherein the filter is impermeable to the stationary phase medium.